No, not hard, just crazy expensive.
>Biology usually works
Under the most basic circumstances. Most startups won't have a business model based on doing PCRs with a master-mix.
>For example I have done over 300 gibson assembly reactions and have taught an intern to do this, he made 50 constructs in 2 months, with time left over for him to biochemically test 25 of them.
Likely at a corporation with money to blow on facilities, equipment, and reagents.
We are talking bare-bones start-up here.
>I'm doing something relatively difficult literally in my garage right now
If you want to make impure vitamins and cram them into pills, yeah I guess, but if we are talking even rudimentary biotech level work, then a garage just won't cut it.