It would hurt mail reachability, but are there any useful numbers as to how badly?
Additionally, it seems as though a solution analogous to HSTS might be better than involving DNSSEC. An SMTP server could advertise that it should be connected to only over TLS - information that could be cached by the sending system.
There is still a MITM problem on 1st connection, but that's an issue for many systems and avoids trusting DNSSEC.