The pilots he was training were coming from an aircraft with a V1 around 80 knots fully laden. V1 for a 737-400 is around 120 knots so if he triggers an aft cargo door warning at 100 knots then the pilots should abort the takeoff.
He was doing this deliberately to emphasise to the trainees this particular difference between their old aircraft and the new one.
This interpretation is at least consistent, but I'm not sure whether it's correct or not.