Define "real DOS machine".
But I would give you my definition: something with ISA slot so you can hear that awful 2.0 stereo SB Pro-compatible with a hiss what could be almost parseltongue. Video card of choice.
So basically anything between 386sx to P3 Tualatin and some rare and weird cases even P4 and AMD Athlon.
https://theretroweb.com/motherboards?page=1&itemsPerPage=24&...