No, but almost everyone ignored it and it never matured out of Candidate Recommendation:

> Efforts to standardize Do Not Track by the W3C in the Tracking Preference Expression (DNT) Working Group reached only the Candidate Recommendation stage and ended in September 2018 due to insufficient deployment and support. [...] Despite supporting it in its Chrome web browser, Google did not implement support for DNT on its websites, and directed users to its online privacy settings and opt-outs for interest-based advertising instead. The Digital Advertising Alliance, Council of Better Business Bureaus and the Direct Marketing Association does not require its members to honor DNT signals.

Source: https://en.wikipedia.org/wiki/Do_Not_Track