I can think of one email client with ~15 million users that does that.
Such pixel are only used by newsletter or shaddy spam senders.
Good that Outlook and other mail apps blocks third party pictures by default.
And of course, a lot of email clients only open such images on demand anyway.
http://blog.mailchimp.com/how-gmails-image-caching-affects-o...
Since Google's goal with this was clearly to defeat tracking, I would strongly expect the former, but I can't back this up.